一种基因组dna的提取方法

Method for extracting genome DNA

Abstract

The invention discloses a method for extracting genomic DNA. A cell lysate (5-50mmol/L Tris.Cl(pH8.0), 0.2-20mmol/L EDTA(pH8.0),0.1-10 percent SDS) is added into a sample, and a sample cell is lysed; RNase digestion is performed; the sample is cooled, a protein precipitation liquid (1-10mol/L (NH4)2SO4 or 1-10mol/L NH4Ac) is added, and protein is removed through centrifugal precipitation; and a supernatant fluid after the centrifugation is transferred into 100 percent isopropanol, and the DNA is centrifugally precipitated. Compared with the prior art, the method can quickly obtain high-purity high-quality genomic DNA, the whole process only needs 30 minutes, and an obtained DNA fragment is about between 50 and 500kb. And the method has the advantages of simple operation, no need of complicated equipment, low cost, cleanliness, no toxicity, and no harm to users and the environment.
本发明公开了一种基因组DNA的提取方法,在样本中加入细胞裂解液(5~50mmol/L Tris.Cl(pH8.0),0.2~20mmol/L EDTA(pH8.0),0.1~10%SDS),裂解样本细胞;RNase消化;(3)将样品冷却,加入蛋白沉淀液(1~10mol/L(NH4)<sub>2</sub>SO<sub>4</sub>或1~10mol/L NH<sub>4</sub>Ac),离心沉淀去除蛋白;将离心后的上清液转移到100%异丙醇中,离心沉淀DNA。与现有技术相比,本方法能够快速得到高纯度高质量的基因组DNA,整个过程可快至30分钟,得到的DNA片段约为50~500Kb。并且,本方法操作简单、无需复杂设备、花费低廉,清洁无毒,对使用者和环境均无害。

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